Performing test flights to detirmine key characteristics and Vrefs.
When trialing FuelUsage(next post), stall speeds at 2000ft, practical ceilings, elevator trim settings for take off, rotate speeds, minimum cruise settings, emergency descent settings, engines fail glide settings, final approach configs (how far do you want to go??), . . . . it is more significant to vary the weight of the aircraft than to vary the temperature, thus you can perform all your testflights in the same User-defined weather setup if you wish.
If you are really worried about the temperature affect on performance just remember -
- in HOTTER Conditions slightly INCREASE throttle and Vrefs -
- in COLDER Conditions slightly DECREASE throttle and Vrefs.
But again I say - you might as well perform all test trails in MID TEMP conditions. (except IAS:TAS trials)
My choice would be the following: - no wind with these temperature layers (see earlier post for more details on setting up your weather.) format: tempF:dewpointF
___ALT____MID_TEMP
40000____-57:-58
35000____-52:-57
30000____-35:-51
25000____-19:-37
20000______0:-20
15000_____17:-5
10000_____34:10
6000______49:23
3000______54:26
Ground____66:35
Conduct the same testflight in these three scenarios - LIGHTEST, MIDWEIGHT, and HEAVIEST.
To me, LIGHTEST means just you (170lbs) and just enough fuel to ascend to a practical cruise level - turn around descend and land. This varies from aircraft to aircraft. Example: the Mooney Bravo = 85 lbs fuel or 15%. The Boeing747 = 19150 lbs fuel or 5%.
To me, HEAVIEST means full fuel and enough payload to bring you up to Max Gross weight.
MIDWEIGHT would be right in between.
The natural progression of testing and detirmining would go something like this:-
1) THE PRACTICAL CEILING
Jump into a fully loaded aircraft and climb until it becomes ridiculous to climb much higher. It is your personal preference. I would say the Practical Ceiling for the Douglas DC3 is 16000 ft, - but if you want spend another half an hour to climb to 17000 , - that's your choice. 
(** remember when pushing altitude limits you should increase RPM higher than normal climb or cruise RPM **)
2) Find the LIGHTEST config'. Put about 15% fuel in aircraft (with 170lbs pilot) and fly up to practical ceiling and back to land. Work out how much fuel you used. Define LIGHTEST, MIDWEIGHT, and HEAVIEST weights.
3) Fly up to 2000ft and test for stall speeds (clean config')
4) Decide and trial rotate and Take Off configs and speeds.(elevator trim also)
example: Douglas DC3
__________T.O.lbs___Stall___T.O.Flaps___T.O.Elev___T.O.RPM_____Throttle"____Rotate
LIGHTEST___16750_____70_____none_______4.2%____2400(77%)_____40_________90
MIDWEIGHT_21475_____75_____none_______4.7%____2500(85%)_____42_________95
HEAVIEST___26200_____80_____none_______5.2%____2600(92%)_____44________100
5) Trial and chart CLIMB SETTINGS and PERFORMANCE
example: The Douglas DC3
LIGHTEST 37"throttle
_ALT____IAS____RPM_____fpm___Fuel(lbs)_Dist
_2000___110___2250____1100_____37_______2.3
_4000___108___2250____1000_____60_______6.1
_6000___106___2250_____950_____86_______9.5
_8000___104___2250_____850____112______13.8
10000___102___2250_____800____141______19.1
12000___100___2250_____750____172______24.2
14000____98___2275_____600____211______30.0
16000____96___2325_____600____247______37.0
MIDWEIGHT 39"throttle
_ALT____IAS____RPM_____fpm___Fuel(lbs)_Dist
_2000___115___2350_____800_____47_______3.0
_4000___113___2350_____750_____81_______7.7
_6000___111___2350_____700____117______13.2
_8000___109___2350_____650____158______18.5
10000___107___2350_____600____202______25.1
12000___105___2375_____550____251______32.5
14000___103___2425_____500____303______41.9
16000___101___2475_____450____357______51.4
HEAVIEST 41"throttle
_ALT____IAS____RPM_____fpm___Fuel(lbs)_Dist
_2000___120___2450_____650_____70_______4.1
_4000___118___2450_____650____114______10.4
_6000___116___2450_____550____159______16.8
_8000___114___2450_____500____219______25.1
10000___112___2450_____425____283______33.7
12000___110___2500_____400____355______44.4
14000___108___2550_____350____436______56.7
16000___106___2600_____250____524______73.2
6) For some aircraft you could compile a list of approximate mixture settings
NOTE: In COLDER conditions set mixture slightly RICHER - - - - HOTTER = LEANER
example : - Douglas DC3
_ALT____Mixture%
_2000_____64
_4000_____55
_6000_____47
_8000_____40
10000_____34
12000_____29
14000_____25
16000_____22
18000_____20
